Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), eBioscience
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 재고 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
14-2231-85 | - | Thermo Fisher Scientific 14-2231-85 CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), eBioscience 500 ug pk | 재고문의 | pk | 448,000원 | - | 492,800원 | ||
14-2231-82 | - | Thermo Fisher Scientific 14-2231-82 CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), eBioscience 100 ug pk | 재고문의 | pk | 232,000원 | - | 255,200원 | ||
14-2231-81 | - | Thermo Fisher Scientific 14-2231-81 CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), eBioscience 50 ug pk | 재고문의 | pk | 131,000원 | - | 144,100원 |
Applications
Tested Dilution
Publications
Flow Cytometry (Flow)
0.5 µg/test
View 9 publications 9 publications
ELISA (ELISA)
2 µg/mL
Immunoprecipitation (IP)
Assay-Dependent
Functional Assay (Functional)
Assay-Dependent
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat / IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
eBioC9B7W (C9B7W)
Conjugate
Unconjugated Unconjugated Unconjugated
View additional formats
- APC
- APC-eFluor 780
- Brilliant Violet 480
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Request custom conjugation
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_493949
Product Specific Information
Description: The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated alpha/beta-TCR bearing T cells, a subset of gamma/delta-TCR bearing T cells and a subset of NK cells. CD223 is a 70 kDa type I transmembrane protein with a structure that is similar to CD4. However, a soluble form of human CD223 has been detected by ELISA in human serum, and data suggest that mouse CD223 is proteolytically cleaved in the D4 domain. This results in a 54 kDa fragment containing all the extracellular domains, and a 16 kDa fragment containing the intracellular and transmembrane domains. The 54 kDa can remain membrane-associated or be released as soluble CD223.
CD223 binds to MHC class II with higher affinity than CD4, and it is thought that this interaction is involved in the negative regulation of T-cell activation and homeostatic proliferation. Furthermore, CD223 is expressed by CD4+CD25+ regulatory T cells, and it has been suggested that CD223 may be involved in their regulatory function.
Applications Reported: This eBioC9B7W (C9B7W) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and ELISA. (Please use Functional Grade purified eBioC9B7W (C9B7W), Product # 16-2231-85, in functional assays).
Applications Tested: This eBioC9B7W (C9B7W) antibody has been tested by flow cytometric analysis of anti-CD3 and anti-CD28-activated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
This eBioC9B7W (C9B7W) antibody has also been tested by ELISA analysis of supernatant produced from mouse CD223-transfected 3A9 cells. This can be used in ELISA at 2 µg/mL for capture of the secreted form of CD223, together with the polyclonal anti-mouse CD223 (Product # 24-2232) as the detection antibody.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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