Thermo Fisher Scientific CD209 (DC-SIGN) Monoclonal Antibody (eB-h209), eBioscience
인간 CD209 (DC-SIGN)을 인식하는 Rat IgG2a 단일클론 항체로, 흐름세포분석과 면역침강에 적합합니다. HIV 및 ICAM-3 결합 연구에 활용 가능하며, 고순도(>90%)로 제조되어 안정적인 신뢰성 제공합니다.
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Applications
Immunohistochemistry (IHC)
- Not tested
View 1 publication
Flow Cytometry (Flow)
- Tested Dilution: 1 µg/test
View 7 publications
Immunoprecipitation (IP)
- Assay-Dependent
View publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eB-h209 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865035 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Conjugated Formats
Product Specific Information
Description:
The eB-h209 monoclonal antibody reacts with human CD209 (DC-SIGN), a 44 kDa type II transmembrane protein with a C-type lectin binding domain that binds ICAM-3, ICAM-2, and HIV virus. DC-SIGN is preferentially expressed on human dendritic cells and may function as a receptor for capture, trafficking, and transmission of HIV to T cells, supporting primary immune responses. eB-h209 was developed against a C-terminal peptide of human DC-SIGN.
Applications Reported:
Flow cytometric analysis and immunoprecipitation.
Applications Tested:
Tested by flow cytometric analysis of cultured human dendritic cells and peripheral blood cells. Recommended usage ≤1 µg/test (100 µL final volume). Cell count per test typically ranges from 10⁵ to 10⁸ cells. Optimal titration is recommended for best assay performance.
Target Information
This gene encodes a transmembrane receptor known as DC-SIGN, expressed on dendritic cells and macrophages. It plays a key role in innate immunity by recognizing diverse pathogens, including viruses and parasites. The protein comprises three domains: an N-terminal transmembrane domain, a tandem-repeat neck domain, and a C-type lectin carbohydrate recognition domain. The extracellular region functions both as a pathogen recognition receptor and a cell adhesion receptor. The neck domain mediates homo-oligomerization, enabling high-avidity multivalent ligand binding. Variations in the neck domain repeat number affect ligand-binding ability. DC-SIGN is closely related to L-SIGN (GeneID 10332), differing in ligand specificity and tissue distribution. Multiple splice variants exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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