Thermo Fisher Scientific CD209a Monoclonal Antibody (LWC06), eBioscience
마우스 CD209(CIRE/DC-SIGN)을 인식하는 Rat IgG2a 단클론 항체로, WB와 IP에 사용 가능. 비결합형 액상 포맷이며, 0.5 mg/mL 농도로 제공. 4°C 보관, 0.09% sodium azide 포함. DC-SIGN 관련 면역학 연구에 적합.
- 카탈로그번호
- 14-2092-xx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD209a Monoclonal Antibody (LWC06), eBioscience
Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-Dependent | View 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Hamster, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LWC06 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865033 |
Additional Formats
Product Specific Information
The LWC06 antibody was generated by immunization with the recombinant extracellular region of mouse CIRE/DC-SIGN (CD209).
CIRE/DC-SIGN is a 33 kDa type II transmembrane C-type lectin protein expressed on CD8α⁻ dendritic cells and plasmacytoid predendritic cells, and is the closest homologue of human DC-SIGN.
It binds mannose and other carbohydrates in a calcium-dependent manner and may play a role in T-dendritic cell interactions through ICAM family binding.
CIRE/DC-SIGN expression varies by dendritic cell subpopulation and activation state. It is down-regulated in GM-CSF–supplemented spleen-derived dendritic cell cultures.
Unlike human DC-SIGN, which is dendritic cell–specific, CIRE/DC-SIGN mRNA is also detected in B cells.
The LWC06 monoclonal antibody does not cross-react with SIGNR1, SIGNR2, SIGNR3, or SIGNR4.
CD209 protein is sensitive to collagenase treatment.
Applications Reported: Immunoprecipitation (IP) and Western blot (WB).
Applications Tested: Tested in CIRE-transfected CHO cells by IP and WB using reciprocal detection with another CIRE-specific antibody (clone 5H10, Product #14-2091).
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
This gene encodes a transmembrane receptor often referred to as DC-SIGN, expressed on dendritic cells and macrophages.
It functions in innate immunity by recognizing a wide range of pathogens (parasites to viruses).
The protein has three main domains:
- N-terminal transmembrane domain
- Tandem-repeat neck domain
- C-type lectin carbohydrate recognition domain
The extracellular region mediates pathogen recognition and cell adhesion by binding carbohydrate ligands.
The neck domain supports homo-oligomerization, enabling high-avidity binding to multivalent ligands.
Variation in the number of 23-amino-acid repeats in the neck region affects ligand-binding ability.
This gene is closely related to L-SIGN (GeneID 10332) but differs in ligand-binding properties and tissue distribution.
Alternative splicing results in multiple variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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