Thermo Fisher Scientific mTOR Monoclonal Antibody (F11), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
This F11 monoclonal antibody reacts with human, mouse, and rat mammalian target of rapamycin (mTOR). This 289-kDa serine/threonine kinase is activated via phosphorylation at Ser2448 by the PI3K/Akt signaling pathway in response to growth factors, energy, nutrients, and stress. mTOR is highly conserved among eukaryotes and constitutively expressed in immune (T, B, NK, dendritic, mast, and neutrophil cells) and non-immune tissues (e.g., heart).

mTOR exists as two distinct complexes, mTORC1 and mTORC2, with differing sensitivities to rapamycin and phosphorylation targets. Rapamycin inhibits mTOR activity by binding FKBP12, causing dissociation of mTORC1 and mTORC2. This molecule plays a critical role in cell metabolism, survival, dendritic cell maturation and activation, and protein synthesis. Deregulation of mTOR has been implicated in various cancers, and studies suggest a role in Foxp3 expression and regulatory T cell differentiation.

Applications Reported:
Reported for use in immunoprecipitation, immunoblotting (WB), and immunohistology staining of paraffin-embedded tissue sections.

Applications Tested:
Tested by western blot analysis of reduced cell lysates prepared from Jurkat cells. Recommended use at 1–5 µg/mL. Antibody should be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FRAP1 (mTOR) is a serine/threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR/PI3-kinase/AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are used as immunosuppressants and cancer therapeutics. Mutations affecting this gene result in Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.