Thermo Fisher Scientific CD207 (Langerin) Monoclonal Antibody (eBioL31), eBioscience
마우스 CD207(Langerin)을 인식하는 eBioL31 단클론 항체로, 랑게르한스 세포 연구에 적합합니다. Western blot, Flow cytometry, IHC 등 다양한 응용에 사용 가능하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 고순도 정제 및 안정적 저장 조건을 갖추었습니다.
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- 14-2075-xx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD207 (Langerin) Monoclonal Antibody (eBioL31), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/test | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 5 publications |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 8 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioL31 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865031 |
Additional Formats
Product Specific Information
The eBioL31 monoclonal antibody reacts with mouse CD207 (Langerin), expressed in a subset of dendritic cells known as Langerhans cells (LC). CD207 is a 48 kDa C-type lectin transmembrane protein involved in antigen recognition and uptake. LC in the epidermis reduce CD207 expression upon activation and migrate toward lymphatic vessels. Expression of CD207 correlates with Birbeck granule presence, and granule formation has been observed upon CD207 cDNA transfection into fibroblast lines.
Western blotting with eBioL31 detects a 48 kDa band in mouse ear epidermis lysates and CD207-transfected cells. Preliminary data suggest suitability for intracellular flow cytometric analysis using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523). The epitope is located in the extracellular CRD region, allowing detection of both surface and intracellular Langerin. Expression levels may vary among mouse strains.
Applications Reported: Flow cytometry, intracellular staining, immunoprecipitation, Western blotting, and frozen tissue immunohistology.
Applications Tested: Flow cytometry and Western blotting with ≤1 µg/test. Optimal dilution should be determined empirically.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Langerin (CD207) is a type II membrane-associated C-type lectin expressed by Langerhans cells. It recognizes mannose residues via its carbohydrate recognition domain (CRD) and is localized both on the cell surface and intracellularly with Birbeck granules. Transfection of Langerin cDNA into fibroblasts induces membrane structures characteristic of Birbeck granules, indicating its role in membrane superimposition and BG formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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