Thermo Fisher Scientific Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

ELISA (ELISA)

3-5 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

PHF13.6

Immunogen

Purified human PHF-Tau preparation if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Tau,uniProtId:P10636-1,ncbiNodeId:9606,antigenRange:1-758,antigenLength:758,antigenImageFileName:35-5300_Tau_P10636-1_House_mouse.svg,antigenImageFileNamePDP:35-5300_Tau_P10636-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533211

Product Specific Information

This monoclonal antibody recognizes phosphorylation-dependent epitopes of ser-396 on PHF-tau as well as fetal tau, but does not recognize autopsy-derived normal adult tau. Phosphoamino ser-396 is one of the two major immunodominant residues of PHF-tau. PHF 13.6 does not recognize unphosphorylated peptide 390-408 or the same peptide phosphorylated at ser-400, thr-403, or ser-404. PHF 13.6 specifically recognizes the ser-396 phosphorylated peptide and all diphosphorylated peptides containing phosphorylated ser-396. Reactivity was confirmed with human PHF-Tau.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimers diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Picks disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.