Thermo Fisher Scientific Phospho-Tau (Ser396) Monoclonal Antibody (PHF13.6)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This monoclonal antibody recognizes phosphorylation-dependent epitopes of serine-396 on PHF-tau as well as fetal tau, but does not recognize autopsy-derived normal adult tau. Phosphoamino ser-396 is one of the two major immunodominant residues of PHF-tau. PHF13.6 does not recognize unphosphorylated peptide 390–408 or peptides phosphorylated at ser-400, thr-403, or ser-404. It specifically recognizes the ser-396 phosphorylated peptide and all diphosphorylated peptides containing phosphorylated ser-396. Reactivity was confirmed with human PHF-Tau.

Target Information

Tau is a neuronal microtubule-associated protein predominantly found on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules, while the N-terminus binds neural plasma membrane components, suggesting a linker function between the two. Axonal polarity is determined by Tau localization within neurons. Short isoforms allow cytoskeletal plasticity, while longer isoforms contribute to stabilization.

In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building blocks of neurofibrillary lesions in Alzheimer’s disease. Hyper-phosphorylation impairs Tau’s microtubule-binding function, leading to microtubule destabilization and neuronal degeneration. Tau is phosphorylated by several kinases, including GSK-3β, PKA, cdk5, and casein kinase II. Hyper-phosphorylated Tau is also found in other neurodegenerative diseases such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.