Thermo Fisher Scientific Phospho-mTOR (Ser2448) Monoclonal Antibody (MRRBY), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.06 µg)/test
• Publications: View 9 publications

Product Specifications

Additional Formats Available

• eFluor 450
• eFluor 660
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This MRRBY monoclonal antibody recognizes human and mouse mammalian target of rapamycin (mTOR, also known as FRAP or RAFT) when phosphorylated on Ser2448.
mTOR is a serine/threonine kinase that functions as an ATP and amino acid sensor, balancing nutrient availability with cell growth, proliferation, motility, survival, protein synthesis, and transcription. Activated mTOR promotes glycolysis-related enzyme production and nutrient uptake, supporting energy demands for cell growth.

Phosphorylation at Ser2448 occurs via the PI3 kinase/Akt pathway, while Ser2481 is autophosphorylated. Dysregulation of mTOR is associated with tumorigenesis, making it a potential target for cancer therapy.

Applications Reported

• Intracellular staining followed by flow cytometric analysis.

Applications Tested

• Pre-titrated and validated for intracellular staining of normal human peripheral blood cells.
• Recommended usage: 5 µL (0.06 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Staining Protocols

• Protocol A (Two-step, cytoplasmic): Flexible detection for surface and intracellular proteins.
• Protocol B (One-step, nuclear): Best for transcription factor and phospho-protein detection.
• Protocol C (Fixation/Methanol): Optimal for phospho-specific signaling discrimination.
  Refer to Clone Performance Following Fixation/Permeabilization in the BestProtocols section.

Detailed staining procedures:
Staining Intracellular Antigens for Flow Cytometry Protocol – BestProtocols®

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

FRAP1 (mTOR):
A serine/threonine kinase essential for cell growth and proliferation. Abnormal activity in the mTOR/PI3K/AKT pathway is linked to various cancers. FRAP1 is the molecular target of rapamycin and its analogs, used as immunosuppressants and anticancer agents. Mutations in FRAP1 are associated with Smith-Kingsmore syndrome.

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

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