Thermo Fisher Scientific EVA1 Monoclonal Antibody (G9P3-1), PE, eBioscience

Thermo Fisher Scientific EVA1 Monoclonal Antibody (G9P3-1), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.5 µg/test
Publications: - References

Product Specifications

Product Specific Information

The monoclonal antibody G9P3-1 reacts with human and mouse epithelial V-like antigen 1 (EVA1), also known as myelin protein zero-like 2 (MPZL2).
EVA1 is a low-affinity adhesion type I transmembrane glycoprotein with an immunoglobulin V-type domain. It is expressed in epithelial tissues and immature thymocytes at the DN3 stage (CD4⁻, CD8⁻, CD25⁺, CD44⁻). EVA1 plays a role in immature thymocyte development and thymic regeneration.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry of mouse thymocytes. Use ≤ 0.5 µg per test (100 µL final volume). Optimal titration recommended.
Excitation / Emission: 488–561 nm / 578 nm (Blue, Green, Yellow-Green Laser).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Thymus development relies on interactions between thymocytes and stromal components. Epithelial V-like antigen (EVA) is expressed in thymus epithelium and downregulated as thymocytes mature.
EVA is homologous to myelin protein zero and associates with the cytoskeleton in thymus-derived epithelial cells. It mediates cell adhesion via homophilic interaction and is regulated by T cell maturation, suggesting a role in early thymus organogenesis.
The protein includes a V-type domain, two potential N-glycosylation sites, and a serine phosphorylation site for casein kinase 2. Two transcript variants encoding the same protein are known.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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