Thermo Fisher Scientific PAX6 Monoclonal Antibody (13B10-1A10)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Western blot analysis of MA1-109 detects an ~47 kDa protein in whole cell lysates from early passage human B3 lens epithelial cells and in mouse eye lysate.
An additional unknown band at ~36 kDa is also detected in B3 cell lysates.
This antibody may detect additional nonspecific bands in lysates with very low basal PAX6 expression.
Specificity was confirmed in B3 and mouse eye lysates (positive controls) compared to U-2 OS human osteosarcoma and NIH/3T3 mouse embryonic fibroblasts (negative controls).

MA1-109 can also be used for immunofluorescence and flow cytometry analysis of Pax6 in human neural stem cells.

Target Information

This gene encodes paired box gene 6 (PAX6), a human homolog of the Drosophila melanogaster gene prd.
The encoded protein contains both a paired box domain and a homeobox domain, which bind DNA and regulate gene transcription.
PAX6 is expressed in the developing nervous system and eyes.
Mutations in this gene are associated with ocular disorders such as aniridia and Peter’s anomaly.
Alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.