Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), Alexa Fluor 488
Alexa Fluor 488으로 결합된 Nestin 단일클론 항체로 인간 및 생쥐 시료에 반응. 신경줄기세포 및 종양 혈관신생 연구에 활용 가능. 단백질 A 정제, 액상 형태로 4°C 암소 보관. ICC/IF, Western blot, Flow cytometry 등 다양한 응용에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Immunogen | Human nestin protein (residues 1464–1614) |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1.0 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Dry ice (international) |
| RRID | AB_2723399 |
Additional Formats
- Unconjugated (MA1-110)
- Alexa Fluor 555 (MA1-110-A555)
- Alexa Fluor 647 (MA1-110-A647)
- Custom conjugation available upon request
Product Specific Information
MA1-110 has been successfully utilized in Western blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples.
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system and early embryonic neuroepithelial stem cells. It serves as a key marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin antibodies are valuable for:
- Characterizing progenitor cell differentiation
- Enhancing therapeutic potential of neural stem/progenitor cells
- Identifying neuroepithelial tumor cells
- Evaluating angiogenesis in tumor endothelial cells
Nestin expression has also been observed in pancreatic hematopoietic stem cells and proliferating endothelial cells in colorectal cancer tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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