Thermo Fisher Scientific TAP Tag Monoclonal Antibody (22F12-1E3), HRP
TAP 태그 단백질 검출용 HRP 결합 단클론 항체로 Western blot에 최적화됨. TEV 절단 후 TAP 구조의 C-말단 펩타이드 인식. 마우스 IgG1 기반, Protein A 정제, 1 mg/mL 농도. 연구용 단백질 상호작용 분석에 적합.
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Applications
- Western Blot (WB)
Tested Dilution: 1:1,000
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 22F12-1E3 |
| Immunogen | KLH conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage |
| Conjugate | HRP |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.07% Kathon |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536819 |
Additional Formats
Product Specific Information
MA1108-HRP detects the TEV cleavage peptide sequence.
MA1108-HRP has been successfully used for direct detection of TAP in Western Blot.
Target Information
Epitope tags provide a method to localize gene products, study protein topology and complexes, identify associated proteins, and characterize low abundance or poorly immunogenic proteins when protein-specific antibodies are unavailable.
Tandem affinity purification (TAP) enables isolation of TAP-tagged proteins and their interacting partners.
The TAP tag typically includes a calmodulin binding peptide (CBP), a TEV protease cleavage site, and Protein A.
This method is highly useful for analyzing in vivo protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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