Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2), Alexa Fluor 647
Alexa Fluor 647로 표지된 Nestin 단클론 항체(클론 10C2). 인간 및 마우스 시료에서 Western blot, ICC/IF 등에 사용 가능. 신경 줄기세포 및 종양 내피세포 마커로 활용. 단백질 A 정제, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Immunogen | Human nestin protein corresponding to residues 1464–1614 |
| Conjugate | Alexa Fluor™ 647 |
| Excitation / Emission Max | 650 / 671 nm |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1.0 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Dry ice (international) |
| RRID | AB_2723401 |
Additional Formats
Product Specific Information
MA1-110 has been successfully utilized in Western blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It is widely used as a marker for:
- Stem/progenitor cells
- Glioma cells
- Tumor endothelial cells
Nestin serves as an angiogenic marker for evaluating neovascularity in tumors.
Highly specific antibodies to human Nestin aid in:
- Characterizing progenitor cell differentiation
- Enhancing therapeutic potential of neural stem/progenitor cells in CNS disease or injury
- Identifying neuroepithelial tumor cells
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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