Thermo Fisher Scientific Nestin Monoclonal Antibody (10C2)
Neuron 및 stem cell marker로 널리 사용되는 Nestin 단클론 항체(10C2). WB, IHC, ICC/IF, Flow 등 다양한 응용 가능. Human 및 Mouse 반응성. 단일클론 Mouse IgG1, Protein A 정제, 액상 형태. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:4,000 | View 6 publications |
| Immunohistochemistry (IHC) | – | View 11 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:100 | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | View publications |
| Immunocytochemistry (ICC/IF) | 1:100 | View 21 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10C2 |
| Immunogen | Human nestin protein corresponding to residues 1464–1614 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536821 |
Available Conjugated Formats
Product Specific Information
MA1-110 has been successfully utilized in Western Blot, immunocytochemistry, flow cytometry, and immunofluorescence applications in human samples.
It acts as a neuronal stem cell marker and detects a band in Western blot at approximately 250 kDa.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It is widely used as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin serves as a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages, enhancing the therapeutic potential of human neural stem/progenitor cells in CNS disease or injury treatment, and identifying neuroepithelial tumor cells.
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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