Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), APC, eBioscience
인간 CD19 항원을 인식하는 SJ25C1 단클론 항체로 B세포 분석에 적합. APC 형광체 결합으로 레드 레이저(633–647 nm)에서 검출 가능. Flow cytometry용으로 사전 희석 및 검증됨. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific CD19 Monoclonal Antibody (SJ25C1), APC, eBioscience™
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 5 µL (1 µg)/test
- Publications: View 14 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SJ25C1 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1272030 |
Available Formats
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 700
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 496 / 615 / 737 / 805
- Brilliant Violet 605 / 650
- eFluor 450 / 506
- NovaFluor Blue 610-70S / 660-120S / 690
- NovaFluor Red 685 / 725 / 755
- NovaFluor Yellow 590 / 610 / 690 / 755
- PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells throughout development except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, Leu13, and MHC class II, associating with the BCR to mediate signaling, tyrosine phosphorylation, calcium flux, and B cell proliferation.
SJ25C1 and HIB19 antibodies recognize overlapping epitopes.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated for flow cytometry using human peripheral blood cells at 5 µL (1 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on all peripheral B cells. It is also found on certain myeloid leukemia cells. CD19 serves as a key regulator of B cell proliferation and differentiation, forming part of the B cell receptor complex to amplify antigen receptor signaling. Mutations in CD19 cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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