Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), APC, eBioscience
인간 CD16(FcγRIII)을 인식하는 eBioCB16 단클론 항체로, APC 형광체가 결합되어 있습니다. Flow cytometry 분석에 최적화되어 있으며, 633–647 nm 레이저에서 660 nm 방출을 보입니다. NK 세포 및 대식세포 연구에 적합합니다.
- 카탈로그번호
- 17-0168-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), APC, eBioscience
Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), APC, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.06 µg)/test
View 37 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioCB16 (CB16) |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2016591 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC-eFluor 780
- Biotin
- Brilliant UV 661 / 737 / 805
- Brilliant Violet 480
- eFluor 450 / 506
- FITC
- Functional Grade
- NovaFluor Blue / Red / Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright series (436, 600, 645, 702, 780)
- Custom conjugation available
Product Specific Information
The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), a low-affinity receptor for IgG with an apparent molecular weight of 50–80 kDa.
CD16 includes two genes:
- CD16A (FcγRIIIA) – membrane-anchored form in macrophages and NK cells
- CD16B (FcγRIIIB) – GPI-anchored form in neutrophils
Two polymorphisms (NA-1, NA-2) exist for CD16B; NA-2 homozygotes show lower phagocytic capacity.
CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, and triggers ADCC, phagocytosis, degranulation, and proliferation upon activation.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of normal human peripheral blood cells.
Use at 5 µL (0.06 µg) per test in a final volume of 100 µL (10⁵–10⁸ cells/test).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD16 encodes a receptor that recognizes the Fc portion of IgG, mediating clearance of immune complexes and antibody-dependent cytotoxicity.
The FCGR3A gene (CD16A) and FCGR3B gene (CD16B) are located on chromosome 1 and encode receptors expressed on NK cells and neutrophils, respectively.
Mutations in FCGR3A are associated with Immunodeficiency 20, Herpes Zoster, and susceptibility to systemic lupus erythematosus and neonatal neutropenia.
Alternative splicing produces multiple isoforms.
For Research Use Only. Not for use in diagnostic procedures.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.