Thermo Fisher Scientific CD20 Monoclonal Antibody (QCH6A7), APC, eBioscience

Thermo Fisher Scientific CD20 Monoclonal Antibody (QCH6A7), APC, eBioscience™

Applications

• Flow Cytometry (Flow)

Tested Dilution: 1 µg/test
Publications: -

Product Specifications

Additional Formats

• Brilliant UV 661
• Brilliant Violet 421
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody QCH6A7 reacts with mouse CD20, a B cell-specific 32-kDa membrane protein expressed on late pro-B cells through memory B cells. CD20 is not found on hematopoietic stem cells, early pro-B cells, or mature plasma cells. It regulates intracellular calcium and plays a role in B cell proliferation.

CD20 expression begins after CD19 induction and increases during maturation, with most mature B cells expressing CD20. Unlike other B cell antigens, CD20 is not shed or internalized upon antibody binding. Monoclonal antibodies against CD20 are used to deplete B cells and serve as therapeutic targets in B cell lymphomas and autoimmune diseases such as rheumatoid arthritis and type I diabetes.

Staining with clone QCH6A7 provides improved staining intensity compared to clone AISB12 in flow cytometry.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse bone marrow and splenocytes; ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, some T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 associates with lipid rafts and remains on the cell surface after antibody binding, making it an effective target for therapeutic B cell depletion.
Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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