Thermo Fisher Scientific Active/Pro-Caspase 3 Recombinant Rabbit Monoclonal Antibody (SU38-04)
Recombinant rabbit monoclonal antibody recognizing Active/Pro-Caspase 3. Western blot과 IHC(P)에서 높은 특이성과 재현성을 보임. Lot 간 일관성 우수하며, 동물 유래 성분이 없는 포뮬레이션. 세포사멸 연구 및 Caspase 경로 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SU38-04 |
| Immunogen | Recombinant protein within Human Caspase-3 (aa 9–175) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809425 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
This gene encodes a protein belonging to the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases is crucial in the execution phase of apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to form active enzyme dimers.
Caspase-3 cleaves and activates caspases 6, 7, and 9 and is itself processed by caspases 8, 9, and 10. It plays a predominant role in amyloid-beta precursor protein cleavage, associated with neuronal death in Alzheimer’s disease. Alternative splicing results in two transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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