Thermo Fisher Scientific p27 Kip1 Recombinant Rabbit Monoclonal Antibody (SU37-04)
Thermo Fisher Scientific의 p27 Kip1 Recombinant Rabbit Monoclonal Antibody (SU37-04)는 다양한 응용 분야(WB, IHC, ICC, Flow)에 사용 가능하며 높은 특이성과 민감도를 제공합니다. HEK293 발현 시스템 기반으로 생산된 재조합 항체로 일관된 품질과 안정적인 성능을 보장합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SU37-04 |
| Immunogen | Synthetic peptide within Human p27 Kip1 aa 149–198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809423 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates for production.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
p27 Kip1 functions as a negative regulator of G1 phase progression and may mediate TGFβ-induced G1 arrest. It is a key regulator of the cell cycle, inhibiting cyclin E- and cyclin A-CDK2 complexes.
p27 Kip1 forms complexes with cyclin D-CDK4, influencing assembly, stability, and activation depending on its phosphorylation state.
This protein (~27 kDa) undergoes degradation via CDK-dependent phosphorylation and ubiquitination by SCF complexes, enabling transition from quiescence to proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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