Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 14 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• Brilliant UV 395 / 563 / 615 / 737
• Brilliant Violet 480 / 605 / 711
• eFluor 660
• NovaFluor Blue 610-70S / 660-120S / 800
• NovaFluor Red 685 / 710
• NovaFluor Yellow 690 / 810
• PE / PE-Cyanine7 / PE-eFluor 610
• Super Bright 436 / 600 / 645 / 780
• Request custom conjugation

Product Specific Information

This 1RNM44N monoclonal antibody recognizes mouse CD170 (Siglec F), a cell surface lectin of the Ig superfamily that binds sialic acid-containing glycoconjugates. The cytoplasmic domain contains an ITIM motif initiating intracellular signaling upon ligand binding.

Siglec F is mainly expressed on eosinophils and alveolar macrophages, with lower expression on immature myelomonocytic cells. It is a functional ortholog of human Siglec 8 but not expressed on mast cells.

• Recognizes a formaldehyde-fixed epitope
• Reported application: Flow cytometric analysis
• Tested on mouse thioglycolate-elicited peritoneal exudate cells
• Recommended ≤0.125 µg/test (100 µL final volume)
• Optimal titration recommended per assay

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the 488 nm blue laser. Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.

Light Sensitivity:
Protect vial and stained samples from light—tandem dye is photo-sensitive.

Fixation:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333).

Excitation / Emission / Laser:
488 nm / 710 nm / Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

SIGLEC5 is a putative adhesion molecule mediating sialic acid-dependent binding to cells. It binds both α2,3- and α2,6-linked sialic acids, with recognition possibly masked by cis interactions on the same cell surface. Expressed by monocytic/myeloid lineage cells, SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, and bone marrow, and at lower levels in lymph node, lung, appendix, placenta, pancreas, and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early myelomonocytic differentiation stages.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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