Thermo Fisher Scientific CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PerCP-eFluor 710, eBioscience
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카탈로그 번호 | CAS 번호 | 설명 | 상태 | 재고 | 단위 | 판매가 | 할인가 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
46-1702-82 | - | Thermo Fisher Scientific 46-1702-82 CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PerCP-eFluor 710, eBioscience 100 ug pk | 재고문의 | pk | 566,000원 | - | 622,600원 | ||
46-1702-80 | - | Thermo Fisher Scientific 46-1702-80 CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PerCP-eFluor 710, eBioscience 25 ug pk | 재고문의 | pk | 241,000원 | - | 265,100원 |
다른 상품 둘러보기
Applications
Tested Dilution
Publications
Flow Cytometry (Flow)
0.125 µg/test
View 14 publications 14 publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat / IgG2a, kappa
Recommended Isotype Control
Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™
Class
Monoclonal
Type
Antibody
Clone
1RNM44N
Conjugate
PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710
View additional formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- Brilliant UV 395
- Brilliant UV 563
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- eFluor 660
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 800
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Yellow 690
- NovaFluor Yellow 810
- PE
- PE-Cyanine7
- PE-eFluor 610
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 780
- Request custom conjugation
Excitation/Emission Max
482/708 nm View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573723
Product Specific Information
Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells.
This 1RNM44N antibody will recognize a formaldehyde-fixed epitope.
Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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