Thermo Fisher Scientific CD155 Monoclonal Antibody (2H7CD155), PerCP-eFluor 710, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test
• View 2 publications

Product Specifications

Additional Formats

• APC
• FITC
• PE
• PE-Cyanine7
• Super Bright 436
• Request custom conjugation

Product Specific Information

Description:
Monoclonal antibody 2H7CD155 recognizes CD155 (poliovirus receptor), a member of the Ig superfamily. Four isoforms exist, with alpha and delta forms expressed on monocytes, prostate cancer cells, and neuroectodermal or monocytic tumor cell lines. CD155 is also found during mid-gastrulation in embryonic development and plays roles in adhesion via vitronectin and NK cell-mediated tumor killing. CD226 and CD96 are ligands for CD155, and blocking CD155 is under investigation for poliovirus variant prevention.

Applications Reported:
Reported for flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells or human tumor cell line U937. Use at 5 µL (0.5 µg) per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirical determination recommended).

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Recommended filter: 710/50 bandpass (695/40 acceptable alternative). Ensure instrument compatibility.

Light Sensitivity:
Tandem dye is sensitive to photo-induced oxidation. Protect vials and stained samples from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222)
  (100 µL cell sample + 100 µL buffer)
• 1-step Fix/Lyse Solution (Product #00-5333)

Store up to 3 days at 4°C in dark with minimal fluorescence loss. Clone-specific performance should be verified empirically.

Excitation: 488 nm
Emission: 710 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

The encoded CD155 protein is a transmembrane glycoprotein of the immunoglobulin superfamily. Its external domain mediates attachment to extracellular matrix molecule vitronectin, and the intracellular domain interacts with dynein light chain Tctex-1/DYNLT1. This gene is primate-specific and serves as a cellular receptor for poliovirus. Multiple transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

제품 이미지