Thermo Fisher Scientific HSP60 Monoclonal Antibody (LK2), FITC
HSP60 단백질 검출용 FITC 결합 단일클론 항체로 다양한 종에 반응. Western blot, IHC, Flow cytometry 등 다중 응용 가능. 단백질 G 정제, 1 mg/mL 농도, 4°C 암소 보관. 연구용 전용 제품.
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Thermo Fisher Scientific HSP60 Monoclonal Antibody (LK2), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100,000 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bacteria, Bovine, Dog, Chicken, Fish, Guinea pig, Hamster, Human, Insect, Mouse, Non-human primate, Nematode, Plant, Pig, Rabbit, Rat, Yeast |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LK2 |
| Immunogen | Recombinant human HSP60 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931567 |
Additional Formats
- APC (MA5-45112)
- PerCP (MA5-45114)
- Request custom conjugation (Custom Form)
Product Specific Information
0.25 µg/mL of MA5-45113 was sufficient for detection of HSP60 in 10 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody. Detects approximately 60 kDa.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the two genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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