Thermo Fisher Scientific HSP60 Monoclonal Antibody (LK2), APC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:100

-

Flow Cytometry (Flow)

Assay-dependent

-

Product Specifications

Species Reactivity

Bacteria, Bovine, Dog, Chicken, Fish, Guinea pig, Hamster, Human, Insect, Mouse, Non-human primate, Nematode, Plant, Pig, Rabbit, Rat, Yeast

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

LK2

Immunogen

Recombinant human HSP60 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HSP60,uniProtId:P10809-1,ncbiNodeId:9606,antigenRange:383-419,antigenLength:573,antigenImageFileName:MA5-45112_HSP60_P10809-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45112_HSP60_P10809-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

View additional formats

• FITC
• PerCP
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

2.48mM MES/95.64mM phosphate with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931566

Product Specific Information

0.25 µg/mL of MA5-45112 was sufficient for detection of HSP60 in 10 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.|Detects approximately 60kDa.

Target Information

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.