Thermo Fisher Scientific HSP60 Monoclonal Antibody (LK1), PerCP
HSP60 단백질을 인식하는 마우스 단클론 항체로 PerCP 결합 형식 제공. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 단백질 접힘 및 미토콘드리아 단백질 조립 연구에 활용 가능. 1 mg/mL 농도의 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:20,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Fruit fly, Guinea pig, Hamster, Human, Insect, Mouse, Non-human primate, Sheep, Plant, Pig, Rabbit, Rat, Shrew, Xenopus |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LK1 |
| Immunogen | Recombinant human HSP60 (amino acids 383–447, UniProt ID: P10809-1) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931565 |
Product Specific Information
0.05 µg/mL of MA5-45111 was sufficient for detection of HSP60 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody. Detects approximately 60 kDa.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. It is essential for the folding and assembly of newly imported proteins in mitochondria. The gene is adjacent to a related family member, and the region between the two genes functions as a bidirectional promoter. Several pseudogenes are associated with this gene. Two transcript variants encoding the same protein have been identified. Mutations in this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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