Thermo Fisher Scientific HSP60 Monoclonal Antibody (LK1), FITC
HSP60 단백질을 특이적으로 인식하는 FITC 결합 단일클론 항체. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합. 다종 교차 반응성. 단백질 G 정제, 보존제 무첨가, 4°C 암소 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:20,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Flow Cytometry (Flow) | Assay-dependent | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Fruit fly, Guinea pig, Hamster, Human, Insect, Mouse, Non-human primate, Sheep, Plant, Pig, Rabbit, Rat, Shrew, Xenopus |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LK1 |
| Immunogen | Recombinant human HSP60 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931564 |
Product Specific Information
- 0.05 µg/mL of MA5-45110 was sufficient for detection of HSP60 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody.
- Detects approximately 60 kDa.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. The gene is adjacent to a related family member, and the intergenic region functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified. Mutations in this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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