Thermo Fisher Scientific HSP60 Monoclonal Antibody (LK2), PerCP
HSP60 단백질을 검출하기 위한 PerCP 결합 단클론 항체. Western blot, IHC, Flow cytometry 등 다양한 응용 가능. 광범위한 종 반응성과 높은 특이성 제공. 연구용으로만 사용.
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Thermo Fisher Scientific HSP60 Monoclonal Antibody (LK2), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:100 |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bacteria, Bovine, Dog, Chicken, Fish, Guinea pig, Hamster, Human, Insect, Mouse, Non-human primate, Nematode, Plant, Pig, Rabbit, Rat, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | LK2 |
| Immunogen | Recombinant human HSP60 (UniProt ID: P10809-1, antigen range: 383–419, antigen length: 573) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931568 |
Additional Formats
Product Specific Information
0.25 µg/mL of MA5-45114 was sufficient for detection of HSP60 in 10 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using goat anti-mouse IgG as the secondary antibody. Detects approximately 60 kDa.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. It is essential for the folding and assembly of newly imported proteins in mitochondria. The gene region acts as a bidirectional promoter between two related family members. Several pseudogenes have been identified, and two transcript variants encoding the same protein are known. Mutations in this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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