Thermo Fisher Scientific 5-Hydroxymethylcytosine Recombinant Rabbit Monoclonal Antibody (RM236)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

0.1-1 µg/mL

-

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

-

Flow Cytometry (Flow)

1-5 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

0.1-1 µg/mL

-

Immunoprecipitation (IP)

0.2-2 µg/mL

-

Dot blot (DB)

0.2-1 µg/mL

-

Product Specifications

Species Reactivity

Chemical

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM236

Immunogen

BSA-conjugated 5-hydroxymethylcytosine

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4, with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2665308

Product Specific Information

This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.