Thermo Fisher Scientific 5-Hydroxymethylcytosine Recombinant Rabbit Monoclonal Antibody (RM236)
5-hydroxymethylcytosine를 특이적으로 인식하는 재조합 토끼 단클론 항체로 높은 특이성과 민감도를 제공. IHC, ICC, Flow, ELISA 등 다양한 응용 가능. 로트 간 일관성과 동물 유래 성분 없음. DNA 메틸화 연구 및 에피제네틱 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| Flow Cytometry (Flow) | 1–5 µg/mL |
| ELISA | 0.1–1 µg/mL |
| Immunoprecipitation (IP) | 0.2–2 µg/mL |
| Dot blot (DB) | 0.2–1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM236 |
| Immunogen | BSA-conjugated 5-hydroxymethylcytosine |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2665308 |
Product Specific Information
This antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA.
No cross-reactivity with non-methylated cytosine and methylcytosine in DNA.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA.
It results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases.
Initially identified in Purkinje neurons, granule cells, and embryonic stem cells, 5-hmC is abundant in brain tissue, especially in regions associated with higher cognitive functions.
Early evidence suggests that 5-hmC may represent an intermediate in a new DNA demethylation pathway converting 5-mC to cytosine.
Although its precise role remains under investigation, recent studies indicate that 5-hmC may play distinct roles from 5-mC, opening new perspectives in epigenetic research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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