Thermo Fisher Scientific H3K79me3 Recombinant Rabbit Monoclonal Antibody (RM157)
H3K79me3 특이적 항체로 Western blot, ELISA, ChIP 등 다양한 응용에 적합. 인간 및 마우스 시료 반응. 재조합 토끼 단클론 항체로 높은 특이성과 일관성 제공. 단백질 A 정제 및 PBS/glycerol 저장용액 구성.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
| ELISA | 0.1–0.5 µg/mL |
| ChIP assay (ChIP) | 1.5 µg / 1×10⁶ cells |
| Luminex (LUM) | 0.1–0.5 µg/mL |
| Peptide Array (Array) | Assay-dependent |
| Dot blot (DB) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM157 |
| Immunogen | Tri-methyl-peptide corresponding to Tri-methyl-Histone H3 (Lys79) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661898 |
Product Specific Information
This antibody specifically recognizes Histone H3 trimethylated at Lysine 79 (H3K79me3).
No cross-reactivity is observed with monomethylated (K79me1) or dimethylated (K79me2) forms, or with other histone H3 methylations.
Recombinant rabbit monoclonal antibodies are generated using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and the best clones are selected for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin–free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it forms nucleosomes that compact and organize DNA.
Histones play essential roles in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes multiple post-translational modifications (e.g., methylation, acetylation, phosphorylation) that influence gene expression and chromatin structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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