Thermo Fisher Scientific 5-Methylcytosine Recombinant Rabbit Monoclonal Antibody (RM231)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

0.5-2 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5-2 µg/mL

-

ELISA (ELISA)

0.1-3 µg/mL

-

Immunoprecipitation (IP)

0.2-2 µg/mL

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Dot blot (DB)

0.5-2 µg/mL

-

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

RM231

Immunogen

BSA-conjugated 5-methylcytosine

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2-7.4, with 1% BSA, 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2665309

Product Specific Information

This antibody reacts to 5-methylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and hydroxymethylcytosine in DNA.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.