Thermo Fisher Scientific Phospho-Histone H2A/H4 (Ser1) Recombinant Rabbit Monoclonal Antibody (RM216)
Phospho-Histone H2A/H4 (Ser1)을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공합니다. Western blot, ICC/IF, ELISA, Luminex 등 다양한 응용에 적합합니다. 로트 간 일관성과 동물 유래 성분이 없는 포뮬레이션을 제공합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| Luminex (LUM) | 0.1–1 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM216 |
| Immunogen | Phospho-peptide corresponding to Phospho-Histone H2A (pSer1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS (pH 7.2–7.4) with 50% glycerol, 1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | –20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661884 |
Product Specific Information
This antibody reacts with Histone H4 or Histone H2A phosphorylated at Serine 1, with no cross-reactivity to other phosphorylated histones.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units called nucleosomes.
The linker histone H1 interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher-order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but contain a palindromic termination element. It is located within the large histone gene cluster on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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