Thermo Fisher Scientific Histone Macro-H2A.1 Recombinant Rabbit Monoclonal Antibody (RM248)
Histone Macro-H2A.1 단백질을 특이적으로 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. WB, ICC/IF, ELISA, ChIP, Luminex 등 다양한 응용에 적합. 높은 특이성과 로트 간 일관성 제공. 인간 및 생쥐 반응성.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| ELISA | 0.2–1 µg/mL |
| ChIP assay (ChIP) | 1.5 µg/1×10⁶ cells |
| Luminex (LUM) | 0.2–1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | RM248 |
| Immunogen | Peptide corresponding to the C-terminus of human Histone macroH2A1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2–7.4, with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2661883 |
Product Specific Information
This antibody reacts to the core histone macro-H2A.1 (Histone macroH2A1) protein, independent of post-translational modifications. No cross-reactivity with other histone proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. Antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
Histones are basic nuclear proteins responsible for nucleosome structure in eukaryotic chromatin. Two molecules of each core histone (H2A, H2B, H3, and H4) form an octamer around which ~146 bp of DNA is wrapped, forming nucleosomes. The linker histone H1 interacts with linker DNA and contributes to chromatin compaction.
This gene, located on chromosome 6, is intronless and encodes a member of the histone H4 family. Transcripts lack polyA tails and contain a palindromic termination element.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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