Thermo Fisher Scientific PKA alpha Monoclonal Antibody (6D2.1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Dot blot (DB)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6D2.1

Immunogen

Synthetic peptide corresponding to residues ESPAQNTAHLDQFERIK of human proteinkinase Ac alpha (PKAc alpha) (aa 32-49). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKA alpha,uniProtId:P17612-1,ncbiNodeId:9606,antigenRange:32-49,antigenLength:351,antigenImageFileName:MA1-19732_PKA_alpha_P17612-1_House_mouse.svg,antigenImageFileNamePDP:MA1-19732_PKA_alpha_P17612-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1090646

Product Specific Information

This antibody reacts with human proteinkinase A (an intracellular antigen) catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

Target Information

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.