Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), Alexa Fluor 488, eBioscience
인간 CD47 단백질을 인식하는 B6H12 클론의 Alexa Fluor 488 결합 단일클론 항체로, 유세포분석(Flow Cytometry)에 최적화됨. 중화능을 가지며 인간 혈액세포 분석에 사용 가능. 488 nm 여기, 519 nm 방출 특성으로 청색 레이저에 적합.
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Thermo Fisher Scientific CD47 Monoclonal Antibody (B6H12), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (1.0 µg)/test | View 10 publications |
| Inhibition Assays (IA) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 488, eBioscience |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B6H12 |
| Conjugate | Alexa Fluor 488 |
| Excitation/Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848420 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 700
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- NovaFluor Yellow 660
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody B6H12 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion through interactions with SIRP and integrins (CD51/CD61, CD41/CD61).
It mediates bidirectional signaling, regulates macrophage phagocytosis, and acts as a receptor for thrombospondin.
- CD47 expression is found in most hematopoietic cells (T cells, B cells, monocytes, platelets, erythrocytes) and also in non-hematopoietic cells.
- This antibody has neutralizing activity.
- Reported for flow cytometric analysis of normal human peripheral blood cells.
- Tested at 5 µL (1.0 µg)/test, suitable for a final volume of 100 µL per sample.
- Excitation: 488 nm, Emission: 519 nm, Laser: Blue Laser.
Target Information
CD47 (Integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with integrins and regulates signaling pathways such as G protein activation.
It functions in calcium signaling upon cell adhesion, serves as a receptor for thrombospondin, and participates in membrane transport and signal transduction.
CD47 binds CD172a (SIRPα) on macrophages, inhibiting phagocytosis of CD47-positive cells.
It plays roles in cell migration, B cell adhesion, T cell activation, chondrocyte mechanotransduction, and neuronal development, especially in synapse-rich brain and retina regions.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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