Thermo Fisher Scientific CD49e (Integrin alpha 5) Monoclonal Antibody (eBioSAM-1 (SAM-1, SAM1)), Alexa Fluor 488, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 2 publications 2 publications

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG2b

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioSAM-1 (SAM-1, SAM1)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• Unconjugated
• Biotin
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10598514

Product Specific Information

Description: The eBioSAM-1 monoclonal antibody reacts with human integrin alpha 5, also known as fibronectin receptor alpha chain, very late activation antigen 5 alpha, and CD49e. Integrins are composed of an alpha chain and a beta chain, which non-covalently associate to form the functional integrin. Integrin heterodimers participate in cell surface-mediated signaling and adhesion functions. Integrin alpha 5 undergoes post-translational cleavage in its extracellular domain to yield disulfide linked light and heavy chains that join with Integrin beta 1 (CD29) to form the fibronectin receptor, also known as the very late activation antigen-5 (VLA-5) complex. Integrin alpha 5 is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated B cells.

Applications Reported: This eBioSAM-1 (SAM-1, SAM1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioSAM-1 (SAM-1, SAM1) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.