Thermo Fisher Scientific LYVE1 Monoclonal Antibody (ALY7), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific LYVE1 Monoclonal Antibody (ALY7), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats:

• Unconjugated
• Biotin
• eFluor 450
• eFluor 570
• eFluor 615
• eFluor 660
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein similar to CD44. LYVE-1 is expressed on lymphatic and liver endothelial cells and some macrophages. It contains a conserved hyaluronan binding domain and functions as a receptor for hyaluronan (HA).
LYVE-1 is a key marker for lymphatic endothelial cells, along with podoplanin, PROX-1, Tie-2, and VEGFR-3. Although LYVE-1 binds HA in vitro, its binding site may be masked in vivo by glycan chains, requiring modification for ligand interaction.

Applications Reported:

• Flow cytometric analysis
• Immunohistologic staining of frozen tissue sections

Applications Tested:

• Immunofluorescence microscopy on mouse intestine (≤2 µg/mL recommended)

Excitation / Emission / Laser:

• 488 nm / 519 nm / Blue Laser

Filtration:

• 0.2 µm post-manufacturing filtered

Target Information

LYVE1 is a major receptor for hyaluronan (HA) on lymph vessel walls. It encodes a 322-residue type I integral membrane glycoprotein with a 212-residue extracellular domain containing a Link module, similar to CD44.
Unlike CD44, LYVE1 colocalizes with HA on lymphatic vessel luminal surfaces and is absent in blood vessels, making it a specific lymphatic marker.
LYVE-1 plays a role in leukocyte and tumor cell trafficking to lymph nodes and serves as a marker for lymphatic endothelium.
Diseases associated with LYVE1 dysfunction include Complete Androgen Insensitivity Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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