Thermo Fisher Scientific CD360 (IL-21 Receptor) Monoclonal Antibody (eBio4A9), Biotin, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio4A9

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466573

Product Specific Information

Description: The IL-21 receptor (IL-21R), also known as CD360, combines with the common gamma-chain to form a functional receptor for IL-21. IL-21R is expressed on several lineages including both resting and activated B, T, natural killer and dendritic cells. Binding of its ligand, IL-21, in these cells results in the activation of the Jak/Stat signal transduction pathway. The biological effects of ligand binding are diverse and depend on the cellular context and differentiation state of the cell. In the mouse, IL-21 binding to IL-21R induces the apoptosis of resting and activated B cells, enhances anti-CD3-mediated proliferation of T cells, and inhibits the maturation of dendritic cells and the IL-15-mediated expansion of NK cells.

Applications Reported: This eBio4A9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio4A9 antibody has been tested by flow cytometric analysis of mouse splenocytes, thymocytes and bone marrow. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a cytokine receptor for interleukin 21 (IL21). It belongs to the type I cytokine receptors, and has been shown to form a heterodimeric receptor complex with the common gamma-chain, a receptor subunit also shared by the receptors for interleukin 2 (IL2) and interleukin 5 (IL5). This receptor transduces the growth promoting signal of IL21, and is important for the proliferation and differentiation of T cells, B cells, and natural killer (NK) cells. The ligand binding of this receptor leads to the activation of multiple downstream signaling molecules, including JAK1, JAK3, STAT1, and STAT3. Knockout studies of a similar gene in mouse suggest a role for this gene in regulating immunoglobulin production. Three alternatively spliced transcript variants encoding the same protein have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.