Thermo Fisher Scientific CD360 (IL-21 Receptor) Monoclonal Antibody (eBio4A9), Biotin, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

The IL-21 receptor (IL-21R), also known as CD360, combines with the common gamma-chain to form a functional receptor for IL-21. IL-21R is expressed on resting and activated B, T, natural killer, and dendritic cells. Binding of IL-21 activates the Jak/Stat signal transduction pathway, with biological effects depending on the cellular context.

In mice, IL-21 binding to IL-21R induces apoptosis of resting and activated B cells, enhances anti-CD3-mediated proliferation of T cells, and inhibits dendritic cell maturation and IL-15-mediated NK cell expansion.

Applications Reported: This eBio4A9 antibody is reported for use in flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes, thymocytes, and bone marrow at ≤0.5 µg/test (100 µL final volume). Cell count may range from 10⁵–10⁸ cells/test. Optimal titration is recommended for best performance.

Target Information

The IL-21 receptor (IL21R) is a type I cytokine receptor forming a heterodimeric complex with the common gamma-chain, shared by IL-2 and IL-5 receptors. It transduces IL-21 growth-promoting signals, essential for proliferation and differentiation of T, B, and NK cells. Ligand binding activates JAK1, JAK3, STAT1, and STAT3. Mouse knockout studies suggest a role in immunoglobulin regulation. Three alternatively spliced transcript variants encoding the same protein have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.