Thermo Fisher Scientific 14-3-3 beta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to the C-terminal region of rat YWHAB conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:14-3-3 beta,uniProtId:P35213-1,ncbiNodeId:10114,antigenRange:246,antigenLength:246,antigenImageFileName:PA1-4647_14-3-3_beta_P35213-1_Rabbit.svg,antigenImageFileNamePDP:PA1-4647_14-3-3_beta_P35213-1_Rabbit_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2218098

Product Specific Information

This antibody is predicted to react with bovine, canine, chicken, human, mouse, non-human primate, Xenopus and zebrafish based on 100% sequence homology.

This antibody is specific for the ~29 kDa 14-3-3 protein. Immunolabeling is blocked by preadsorption of the antibody with the protein used to generate the antibody.

Not recommended for use in paraffin sections.

Target Information

14-3-3, a family of acidic and soluble proteins, highly conserved in amino acid sequences from yeast to mammals, is expressed in all eukaryotic cells. Seven isoforms(beta, gamma, epsilon, eta, zeta, sigma and tau/theta) encoded by seven distinct genes are identified in mammals and forms homo- and hetero- dimeric cup-shaped structures. As 14-3-3 is interacted with more than 100 binding partners, it regulates key proteins involved in various biological processes such as signal trans-duction, cell cycle, transcriptional control, cell proliferation, apoptosis, and ion channel physiology. Most 14-3-3 requires phosphorylation of serine or threonine residues in the target sequence. This protein is abundantly expressed in the brain and has been detected in the cerebrospinal fluid of patients with different neurological disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.