Thermo Fisher Scientific JAML Monoclonal Antibody (eBio4E10 (4E10)), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Not Applicable

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

eBio4E10 (4E10)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_795882

Product Specific Information

Description: The eBio4E10 monoclonal antibody reacts with mouse JAML (junctional adhesion molecule-like protein), also known as AMICA. JAML is a glycosylated membrane protein possessing two Ig-like domains in its extracellular domain. JAML is expressed on polymorphonuclear leukocytes (PMN), and is involved in the transmigration of such cells through epithelial tight junctions via interactions between JAML and epithelial coxsackie and adenovirus receptor (CAR). JAML is also expressed on lymphocyte subsets, such as gammadelta T cell lines, and can provide co-stimulatory signals for cell activation.

Applications Reported: This eBio4E10 antibody has been reported for use in flow cytometric analysis and immunoprecipitation.

Applications Tested: This eBio4E10 (4E10) antibody has been tested by flow cytometric analysis of mouse lysed whole blood. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

AMICA transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.