Thermo Fisher Scientific Plexin-B2 Monoclonal Antibody (eBio3E7 (3E7)), eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Class

Monoclonal

Type

Antibody

Clone

eBio3E7 (3E7)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_763626

Product Specific Information

Description: Plexin-B2 is a member of the Semaphorin receptor family. The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues. Plexin-B1 is a transmembrane protein that can be cleaved coverting the single chain presursor into a heterodimeric receptor. It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos. Sema4D also plays a role in the immune system interacting with CD72. Plexin-B2`s possible role in the immune system has not been characterized.

Applications Reported: This eBio3E7 (3E7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested: This eBio3E7 (3E7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Plexin-B2 is a member of the Semaphorin receptor family. The semaphorin family consists of membrane and secreted proteins involved in attraction and repulsion of neuronal guidance cues. Plexin-B1 is a transmembrane protein that can be cleaved converting the single chain precursor into a heterodimeric receptor. It has been shown to bind Sema4D/CD100 and is involved in axon development in mouse embryos. Sema4D also plays a role in the immune system interacting with CD72. Plexin-B2`s possible role in the immune system has not been characterized.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.