Thermo Fisher Scientific Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eBioscience
마우스 Podoplanin을 인식하는 단클론 항체로, Flow Cytometry, WB, IHC 등 다양한 면역분석에 적합합니다. 높은 순도(>90%)와 낮은 응집도(<10%)를 가지며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 14-5381-8x (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Podoplanin Monoclonal Antibody (eBio8.1.1 (8.1.1)), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 2 publications |
| Immunohistochemistry (IHC) | - | 22 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | - |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 11 publications |
| Flow Cytometry (Flow) | 0.5 µg/test | 21 publications |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Functional Assay | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Hamster, Human, Mouse, Rat |
| Host / Isotype | Syrian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio8.1.1 (8.1.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1210506 |
Additional Formats
- Alexa Fluor 488
- Biotin
- eFluor 660
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Request custom conjugation
Product Specific Information
Description:
The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein expressed in kidney glomerular epithelial cells (podocytes). Podoplanin is also found in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and visceral peritoneum mesothelium. It serves as a potent marker for lymphatic endothelium and is expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth due to respiratory defects and congenital lymphedema caused by lymphatic pattern formation failure.
Applications Reported:
This eBio8.1.1 (8.1.1) antibody is suitable for flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.
Applications Tested:
Tested by flow cytometric analysis of TE-71 cells. Use ≤0.5 µg per test (in 100 µL final volume). Optimal antibody titration is recommended for best assay performance.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Podoplanin (PDPN, T1A, gp38, Aggrus) is a mucin-type 1 glycoprotein (40–43 kDa) expressed in tumors and normal cells, especially lymphatic epithelial cells and follicular dendritic cells. It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of lymphatic endothelium, podoplanin helps maintain podocyte morphology and serves as a ligand for CLEC-2. Its expression correlates with tumor invasiveness and metastasis potential. Podoplanin is involved in cell migration, metastasis formation, and tumor invasion. Dysregulation of podoplanin is associated with diseases such as lymphangioma and subglottic neoplasm and has been proposed as a marker for lung injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.