Thermo Fisher Scientific Human NGF Recombinant Protein
인간 NGF 재조합 단백질로 신경세포 생존 및 분화 연구에 적합. CHO 세포에서 발현된 고순도 단백질(>95%)로 기능적 활성 검증 완료. 다양한 분석(ELISA, WB, IHC, Functional Assay)에 활용 가능. 실험용으로만 사용.
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Thermo Fisher Scientific Human NGF Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | CHO cells |
| Amino Acid Sequence | Human β-NGF, amino acids Ser122–Arg239 (Accession # NP_002497.2) |
| Molecular Weight | 13.2 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2–2 ng/mL. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 40 mM arginine / 40 mM histidine, pH 5.5, with 5% mannitol, 150 mM NaCl, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Nerve Growth Factor (NGF) influences the survival and differentiation of a variety of neuronal and non-neuronal cells. NGF binds to specific cell surface receptors with both high and low affinity sites, where only the high affinity receptor mediates signal transduction. Studies show that the high affinity receptor of rat PC12 cells forms a complex of Mr 158,000 with NGF, while the low affinity receptor forms a complex of Mr 100,000. Human and rat NGF receptor genes share >90% homology. NGF exhibits nerve growth stimulating activity and regulates growth and differentiation of sympathetic and sensory neurons. Mutations in the NGF gene are linked to hereditary sensory and autonomic neuropathy type 5 (HSAN5), and dysregulation of NGF expression is associated with allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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