Thermo Fisher Scientific Human CD200 His-tag Recombinant Protein
Human CD200 His-tag Recombinant Protein은 HEK293 세포에서 발현된 고순도 재조합 단백질입니다. CD200R 결합 활성 확인용으로 WB, IHC, ELISA 등 다양한 분석에 적합합니다. ≥98% SDS-PAGE 순도, 낮은 엔도톡신 수준, 연구용으로 최적화된 제품입니다.
- 카테고리
- Protein
- 판매단위
- pk
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | HEK293 cells |
| Amino Acid Sequence | Human CD200, amino acids Met1–Gly232 (Accession # NP_005935.4) with a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 24 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥98% (SDS-PAGE), ≥90% (SEC-HPLC) |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Immobilized Human CD200 His-tag Recombinant Protein at 2 µg/mL (100 µL/well) binds CD200R Protein, Human, Recombinant (His & hFc Tag), EC50 = 5–28 ng/mL |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
Reconstitution:
Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CD200 is a type-1 membrane glycoprotein containing two immunoglobulin domains and belongs to the immunoglobulin superfamily. It is a broadly distributed cell surface glycoprotein that interacts with a receptor on myeloid cells (CD200R), implicated in locally preventing macrophage activation. Homologues of CD200 have been identified in many herpesviruses and poxviruses. Studies in mouse and rat suggest CD200 regulates myeloid cell activity and delivers inhibitory signals for macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants encode different isoforms of CD200. Diseases associated with CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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