Thermo Fisher Scientific Influenza A H1N1 (A/California/04/2009) Neuraminidase His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Virus

Expression System

Insect cells

Amino acid sequence

Influenza A virus H1N1 (A/California/04/2009) neuamindase, amino acids His36-Lys469 (Accession # ACP41107.1) with an N-terminal vasodilator-stimulated phosphoprotein tetramizeration domain and a C-terminal His-tag

Tag

His-tag

Molecular weight

53.6 kDa

Class

Recombinant

Type

Protein

Purity

>90% by SDS-PAGE

Endotoxin concentration

<1.0 EU/µg

Activity

Measured by its ability to cleave a fluorogenic substrate, 2`-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. The specific activity is > 8, 000 pmoles/min/µg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

20mM tris, pH 7.5, with 10% glycerol, 5% mannitol, 150mM NaCl, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expiration Date: Expires one year from date of receipt when stored as instructed.

Target Information

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin. The distinct antigenic properties of the new swine influenza virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.