Thermo Fisher Scientific Influenza A H1N1 (A/California/04/2009) Neuraminidase His-tag Recombinant Protein
Thermo Fisher Scientific의 H1N1 Neuraminidase 재조합 단백질로, Insect cells에서 발현된 His-tag 형태입니다. ELISA, WB, IHC 등 다양한 실험에 사용 가능하며, >90% 순도와 높은 효소 활성을 보유합니다. 연구용으로만 사용되며, 안정적인 보관 및 재구성 지침 제공.
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Applications
- Western Blot (WB): Assay-dependent
- Immunohistochemistry (IHC): Assay-dependent
- ELISA: Assay-dependent
- Functional Assay: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Virus |
| Expression System | Insect cells |
| Amino Acid Sequence | Influenza A virus H1N1 (A/California/04/2009) neuraminidase, amino acids His36-Lys469 (Accession # ACP41107.1) with an N-terminal vasodilator-stimulated phosphoprotein tetramerization domain and a C-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 53.6 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >90% by SDS-PAGE |
| Endotoxin Concentration | <1.0 EU/µg |
| Activity | Cleaves fluorogenic substrate 2`-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid; specific activity >8,000 pmoles/min/µg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Storage Buffer | 20 mM Tris, pH 7.5, with 10% glycerol, 5% mannitol, 150 mM NaCl, 5% trehalose, 0.01% Tween 80 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient |
Product Specific Information
- Reconstitution: Dissolve in sterile double distilled water to ≤0.2 mg/mL. Aliquot and store under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
- Expiration Date: One year from receipt when stored as instructed.
Target Information
Influenza A virus is a major public health threat, responsible for over 30,000 deaths annually in the USA. In 2009, a novel swine-origin influenza A (H1N1) virus emerged, spreading globally and declared a pandemic by WHO.
Influenza A virus has 16 possible Hemagglutinin (HA) and 9 Neuraminidase (NA) surface proteins. HA aids viral attachment, while NA facilitates viral release and elicits immune responses.
The 2009 H1N1 strain combined human, swine, and avian influenza gene segments with additional pig H3N2 genes of Eurasian origin. Its distinct antigenic profile limits preexisting human immunity, though partial protection may exist in older populations.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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