Thermo Fisher Scientific CaV3.2 Monoclonal Antibody (N55/10), PerCP
CaV3.2 단백질을 특이적으로 인식하는 mouse monoclonal antibody로, PerCP로 표지되어 있습니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, Human, Mouse, Rat 시료에서 반응합니다. 단백질 G 정제, 1 mg/mL 농도, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N55/10 |
| Immunogen | Fusion protein amino acids 1019–1293 (II–III loop) of human Cav3.2 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931851 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45397 was sufficient for detection of Cav3.2 in 10 µg of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 260 kDa. No cross-reactivity against Cav1.3.
Target Information
This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell. Multiple isoforms exist, either encoded by different genes or generated via alternative splicing. Certain mutations in this gene are associated with childhood absence epilepsy (CAE).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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