Thermo Fisher Scientific CaV3.2 Monoclonal Antibody (N55/10), FITC
CaV3.2 단백질을 특이적으로 인식하는 FITC 결합 단일클론 항체. Western blot, IHC, ICC 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. 단백질 G 정제, 4°C 암소 보관. 연구용 전용.
- 카탈로그번호
- MA545396
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N55/10 |
| Immunogen | Fusion protein amino acids 1019–1293 (II–III loop) of human CaV3.2 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS (pH 7.4) with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931850 |
Additional Formats Available
- Unconjugated (MA5-27656)
- APC (MA5-45395)
- PE (MA5-45398)
- PerCP (MA5-45397)
- Custom conjugation upon request
Product Specific Information
1 µg/mL of MA5-45396 was sufficient for detection of CaV3.2 in 10 µg of HEK cell lysate expressing CaV3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 260 kDa. No cross-reactivity against CaV1.3.
Target Information
This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell. Multiple isoforms exist due to alternative splicing. Certain mutations in this gene are associated with childhood absence epilepsy (CAE).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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