Thermo Fisher Scientific CaV3.2 Monoclonal Antibody (N55/10), APC
CaV3.2 단백질을 인식하는 마우스 단일클론 항체로 APC 형광 표지된 제품입니다. Western blot, IHC, ICC, IP 등 다양한 면역 분석에 사용 가능하며 Human, Mouse, Rat 반응성을 보입니다. 단백질 G 정제, 4°C 보관, 연구용 전용.
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Thermo Fisher Scientific CaV3.2 Monoclonal Antibody (N55/10), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N55/10 |
| Immunogen | Fusion protein amino acids 1019–1293 (II–III loop) of human CaV3.2 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931849 |
Additional Formats
- Unconjugated (MA5-27656)
- FITC (MA5-45396)
- PE (MA5-45398)
- PerCP (MA5-45397)
- Request custom conjugation available
Product Specific Information
1 µg/mL of MA5-45395 was sufficient for detection of CaV3.2 in 10 µg of HEK cell lysate expressing CaV3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 260 kDa. No cross-reactivity against CaV1.3.
Target Information
This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio.
The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell.
There are multiple isoforms of each of the proteins in the complex, either encoded by different genes or the result of alternative splicing of transcripts. Alternate transcriptional splice variants encoding different isoforms have been characterized for this gene.
Studies suggest certain mutations in this gene lead to childhood absence epilepsy (CAE).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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