Thermo Fisher Scientific Caspase 7 Monoclonal Antibody (20F3), eBioscience

Applications and Tested Dilution

• Application: Western Blot (WB)
• Tested Dilution: 0.5–5 µg/mL
• Publications: [References not provided]

Product Specifications

Product Specific Information

The monoclonal antibody 20F3 recognizes human Caspase-7, also known as mch3 and ICE-LAP3, a member of the cysteine-requiring aspartate protease family involved in apoptosis. Along with caspases 3 and 7, it functions as an effector or executioner caspase activated by cleavage through caspase-3, -6, -8, or granzyme B.

Caspase-7 mediates the demolition phase of apoptosis by cleaving PARP and other caspase-3 substrates. This antibody detects both full-length (p35) and active (p20) Caspase-7 forms under reducing SDS-PAGE conditions.

• Applications Reported: Immunoblotting (WB)
• Applications Tested: Tested on reduced Jurkat cell lysates at 1–5 µg/mL. Optimal dilution should be determined experimentally.
• Note: A nonspecific band (~58 kDa) may appear in some lysates.
• Purity: >90% (SDS-PAGE)
• Aggregation: <10% (HPLC)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Caspase-7 belongs to the cysteine protease family, divided into apoptotic and inflammatory subfamilies. While inflammatory caspases are linked to immune responses, apoptotic caspases regulate programmed cell death. Caspase-7 functions as an executioner caspase, activated by initiator caspases, and cleaves cellular substrates leading to apoptosis.

It was identified by homology with caspases 1 and 3, and the C. elegans protein CED-3. Alternative splicing of Caspase-7 mRNA produces three isoforms. Caspase-7 activity can be inhibited by XIAP expression. Diseases associated with CASP7 include Type 1 Diabetes Mellitus 17 and Aleutian Mink Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.