Thermo Fisher Scientific Caspase 7 Monoclonal Antibody (20F3), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

20F3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1633375

Product Specific Information

Description: The monoclonal antibody 20F3 recognizes human caspase-7, also known as mch3 and ICE-LAP3. It is a member of the cysteine-requiring aspartate protease family, which plays critical roles in cell suicide known as apoptosis. Along with caspases 3 and 7, this member is categorized as an effector or executioner caspase. It must first be activated via cleavage by capase 3, 6, or 8 or by granzyme B. As an effector molecule, caspase-7 is responsible for the demolition phase of apoptosis in that it cleaves PARP and other molecules also cleaved by caspase-3.

This monoclonal antibody can be used to identify both the full-length (p35) and active form (p20) of caspase-7 under reducing conditions by SDS-PAGE.

Applications Reported: This 20F3 antibody has been reported for use in immunoblotting (WB).

Applications Tested: This 20F3 antibody has been tested by immunoblot analysis of reduced Jurkat cell lysates. This can be used at 1-5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

The presence of a nonspecific band at approximately 58 kDa may be present in some lysates.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Caspase 7 is part of a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase 7 is an executioner caspase that was identified based on its homology with caspases 1 and 3, as well as the C. elegans cell death protein CED-3. Alternative splicing of Caspase-7 mRNA results in the production of 3 distinct isoforms. Caspase-7 activity can be directly inhibited by XIAP expression. Alternative splicing results in four transcript variants, encoding three distinct isoforms of Caspase 7. Diseases associated with CASP7 include Type 1 Diabetes Mellitus 17 and Aleutian Mink Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.