Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• APC (17-9924-82)
• Biotin (13-9924-81)
• Functional Grade (16-9924-82)
• PE (12-9924-82)
• PE-Cyanine7 (25-9924-82)
• Super Bright 436 (62-9924-82)
• Request custom conjugation

Product Specific Information

The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. TLR family members are type I transmembrane proteins characterized by extracellular leucine-rich repeats and a cytoplasmic domain homologous to the IL-1 receptor. TLR4 associates with MD-2 and CD14 to form a complex responsible for LPS recognition and signaling.

In mice, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages and is downregulated upon LPS stimulation. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, which are hyporesponsive to LPS.

MTS510 co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), preferentially binding to TLR4 associated with MD-2. In contrast, UT41 binds TLR4 regardless of MD-2 association.

Applications Reported

• Flow cytometric analysis
• Immunoprecipitation
• Immunohistology of frozen tissue sections
• Inhibition of LPS-induced cytokine production

For functional assays, use Functional Grade purified MTS510 (Product #16-9924-81).

Applications Tested

Tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. Recommended ≤1 µg/test (in 100 µL final volume). Optimal titration recommended per assay.

Purity and Quality

• Purity: >90% (SDS-PAGE)
• Aggregation: <10% (HPLC)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, including Toll-like receptors (TLRs). TLRs recognize pathogen-associated molecular patterns (PAMPs) and link innate and adaptive immunity. TLR4, the major receptor for lipopolysaccharide (LPS), requires MD-2 binding for maximal response. The LPS response specificity depends on MD-2 species; for example, human MD-2 in mouse cells can alter TLR4 response to LPS analogs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.