Thermo Fisher Scientific TLR4/MD-2 Complex Monoclonal Antibody (MTS510), eBioscience
마우스 TLR4/MD-2 복합체를 인식하는 MTS510 단클론 항체로, 선천면역 및 염증 연구에 적합. Flow cytometry, IP, IHC(F) 등 다양한 응용 가능. 0.5 mg/mL 농도의 액상 제품으로 4°C 보관. Affinity chromatography로 정제됨.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 4 |
| Immunohistochemistry (IHC) | - | 3 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 1 |
| Immunocytochemistry (ICC/IF) | - | 2 |
| Flow Cytometry (Flow) | 1 µg/test | 8 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| Neutralization (Neu) | Assay-Dependent | 14 |
| Functional Assay (Functional) | Assay-Dependent | 4 |
| Inhibition Assays (IA) | - | 3 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MTS510 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_468616 |
Additional Formats
- APC (17-9924-82)
- Biotin (13-9924-81)
- Functional Grade (16-9924-82)
- PE (12-9924-82)
- PE-Cyanine7 (25-9924-82)
- Super Bright 436 (62-9924-82)
- Request custom conjugation
Product Specific Information
The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. TLR family members are type I transmembrane proteins characterized by extracellular leucine-rich repeats and a cytoplasmic domain homologous to the IL-1 receptor. TLR4 associates with MD-2 and CD14 to form a complex responsible for LPS recognition and signaling.
In mice, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages and is downregulated upon LPS stimulation. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, which are hyporesponsive to LPS.
MTS510 co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), preferentially binding to TLR4 associated with MD-2. In contrast, UT41 binds TLR4 regardless of MD-2 association.
Applications Reported
- Flow cytometric analysis
- Immunoprecipitation
- Immunohistology of frozen tissue sections
- Inhibition of LPS-induced cytokine production
For functional assays, use Functional Grade purified MTS510 (Product #16-9924-81).
Applications Tested
Tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. Recommended ≤1 µg/test (in 100 µL final volume). Optimal titration recommended per assay.
Purity and Quality
- Purity: >90% (SDS-PAGE)
- Aggregation: <10% (HPLC)
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, including Toll-like receptors (TLRs). TLRs recognize pathogen-associated molecular patterns (PAMPs) and link innate and adaptive immunity. TLR4, the major receptor for lipopolysaccharide (LPS), requires MD-2 binding for maximal response. The LPS response specificity depends on MD-2 species; for example, human MD-2 in mouse cells can alter TLR4 response to LPS analogs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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