Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), eBioscience

Thermo Fisher Scientific CD284 (TLR4) Monoclonal Antibody (HTA125), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). TLR4, a member of the Toll family, is a type I transmembrane protein involved in innate immunity and inflammation. It forms a complex with MD-2 and CD14 for LPS recognition and signaling. TLR4 is expressed on peripheral blood monocytes and shows variable expression among donors.

For optimal staining of TLR4, use the lysed whole blood protocol rather than Ficoll-gradient prepared cells, as the latter reduces staining intensity.

Applications Reported: Flow cytometric analysis, immunoprecipitation, and blocking of LPS-induced cytokine production.
Applications Tested: Flow cytometric analysis of normal human peripheral blood cells (≤1 µg/test).
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR4 is a Toll-like receptor involved in pathogen recognition and activation of innate immunity. It is expressed mainly in placenta and myelomonocytic leukocytes. TLR4 participates in LPS-induced signal transduction with CD14 and LY96. Mutations in TLR4 affect LPS responsiveness. It is expressed by peripheral blood monocytes, a subset of B-cells, and placenta. TLR4-deficient mice show increased susceptibility to Gram-negative sepsis.

For Research Use Only. Not for use in diagnostic procedures.

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