Thermo Fisher Scientific Caspase 8 Monoclonal Antibody (1H10), eBioscience

Applications and Tested Dilution

• Application: Western Blot (WB)
• Tested Dilution: 1–5 µg/mL
• Recommended Use: Optimize dilution for specific assay conditions.

Product Specifications

Product Specific Information

The monoclonal antibody 1H10 recognizes human Caspase-8 (also known as FLICE or MACH), a cysteine-aspartate protease involved in Fas and TNF-α mediated apoptosis. Upon receptor activation, FADD recruits caspase-8 to form a complex that triggers its cleavage and activation. Active caspase-8 (p18/p10 heterotetramer) subsequently activates downstream caspases (-3, -4, -6, -7, -9, -10).

The 1H10 antibody detects the p55 and p53 proforms, as well as the p43 and p18 cleaved forms. Under reducing conditions, an additional ~35 kDa band may appear (possibly non-specific), which is absent under nonreducing conditions.

• Applications Reported: Immunoblotting (WB)
• Applications Tested: Validated in cell line lysates for WB (1–5 µg/mL recommended)

Target Information

Caspase 8 (CASP8) is a key enzyme in the apoptotic signaling pathway. It exists as an inactive proenzyme that undergoes proteolytic activation to form an active heterodimer. Caspase 8 interacts with FADD through its death effector domain (DED) and subsequently activates downstream caspases such as caspase 9, committing the cell to apoptosis.

Caspase 8 has been implicated in neurodegenerative diseases such as Huntington’s disease, where it is detected in affected brain regions. Overexpression of Caspase 8 induces apoptosis, which can be inhibited by ICE family-specific inhibitors. Multiple alternatively spliced isoforms have been identified, though not all are fully characterized.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.